Lab Test To Consider For A Missing Period
- Follicle-stimulating hormone
- Fasting insulin
- Glycated hemoglobin , fasting glucose or glucose challenge
Will you need all of these? Maybe. Maybe not. This is where an experienced clinician can help.
I know that many women experience resistance to their request to have comprehensive hormone testing. I would urge you to seek out a functional medicine practitioner, a naturopathic physician, a reproductive endocrinologist, or a gynecologist. I know it can be super frustrating to try to find a provider that will help you but there are many amazing practitioners out there who can and will help you get the labs you need.
Also dont let a doctor convince you that a missing period doesnt matter because youre not interested in baby-making at the moment. Amenorrhea is a sign of an underlying issue that needs to be addressed, whether youre trying to conceive or not. The quality of care you receive should not be dependent on your whether or not you want to have a baby at this moment.
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Do T4 Test To Check If You Have An Overactive Thyroid
A T4 test helps to check the level of thyroxine hormone in your blood. If your T4 level is high or low, it indicates thyroid disorders. When there is a hormonal imbalance, you tend to feel fatigued and notice unexplained weight gain.
This test can be used to assess both hyperthyroidism and hypothyroidism. While high T4 levels may indicate hyperthyroidism, hypothyroidism is diagnosed with high T4 levels . The normal T4 levels in an individual range between 5.0-11.0 g/dL.
How To Test For Menopause
When it comes to a menopause test, sometimes you can have a blood test to measure the level of certain hormones but it depends on a few factors, such as age and the medication you are taking.
Women under 45 with menopausal symptoms can have a blood test measuring their follicle stimulating hormone . While this can be useful even this can be quite inaccurate, especially if youre taking medication such as the combined contraceptive pill or progesterone-only pill.
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Letsgetchecked Female Hormone Test
Price: $$$
A subscription may be appealing if youre interested in regularly monitoring your hormone levels over time. With LetsGetChecked, you can also save money by signing up for their trimonthly shipments.
After registering your testing kit, youll need to collect a finger-prick blood sample on the third day of your menstrual cycle before 10 a.m. Additionally, you cant take the test if youre using hormonal birth control. After collecting your sample, you have to mail it the same day using the prepaid envelope.
Within 2 to 5 days, you can view your results from your secure online account. A dedicated team of nurses is also available around the clock to discuss them with you.
Why Do I Need An Estrogen Test

You may need an estradiol test or an estrone test if you:
- Are having trouble getting pregnant
- Are a woman of childbearing age who is not having periods or having abnormal periods
- Are a girl with early or delayed puberty
- Have symptoms of menopause, including hot flashes and/or night sweats
- Have vaginal bleeding after menopause
- Are a boy with delayed puberty
- Are a man showing female characteristics, such as the growth of breasts
If you are pregnant, your health care provider may order an estriol test between the 15th and 20th week of pregnancy as part of a prenatal test called a triple screen test. It can find out if your baby is at risk for a genetic birth defect such as Down syndrome. Not all pregnant women need to get an estriol test, but it is recommended for women who have a higher risk of having a baby with a birth defect. You may be at a higher risk if you:
- Have a family history of birth defects
- Are age 35 or older
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When To Test Insulin
If youre trying to get to the bottom of a PCOS diagnosis, you will need to evaluate insulin. If you have a family history of diabetes or your clinician suspects insulin dysregulation, this is a test they will likely order.
Typically, insulin is tested while fasting along with blood glucose. Because of this, the test is commonly done first thing in the morning.
It is recommended that biotin be stopped at least 72 hours before testing.
Fasting insulin is a good place to start, but you may need to do a glucose challenge as well. Finding out what your insulin looks like after you eat can also be helpful. Your doctor will help determine the right tests for you.
Testing + Symptoms = Diagnosis
Both lab tests and symptom charting are not necessarily 100% accurate.
The problem with menopause is that it occurs over a period of several months .
This time is a period of great fluctuation, both in terms of your symptoms and in terms of your lab tests and hormones.
Your hormones and prohormones may fluctuate wildly during this time which means that checking just one time may not necessarily be accurate.
As a woman is going through the menopause transition its not uncommon for her body to attempt to menstruate.
This attempted menstruation may cause small to large rises in hormone levels which may alter your symptoms.
Because of this, you dont want to rely heavily on one form of diagnostic tool over another.
Instead, its best to combine both your symptoms and use them in conjunction with your lab tests .
This is especially important if you are suffering from early menopause.
Women who suffer from early menopause may be pushed aside as having depression as opposed to true ovarian failure/menopause.
The good news is that it is easily testable and that you can be sure what is happening in your body with a couple of lab tests.
For instance:
During menopause, we know that your estradiol levels and progesterone levels WILL fall dramatically.
When in doubt, make sure to ask your doctor for the lab tests listed above as they may help to explain what is happening in your body.
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Do All Patients Need Blood Tests For Hormone Levels
Dr Aziz-Scott: Although treatment is bespoke to you, patients will follow a similar patient journey, and in the initial consultation, blood tests for hormone levels are required for all patients. In some cases, women may come to the clinic with blood tests conducted externally by their GP, or another healthcare professional.
We do see patients that have had a Dried Urine Test for Comprehensive Hormones . The DUTCH test is a urinary assessment of hormones and can provide a great deal of useful information about the different hormone systems in the body. It is very useful in assessing how estrogen is metabolised and this is a very important factor to consider once you are on BHRT as certain estrogen metabolites can be toxic and increase breast cancer risk.
Depending on a patients individual situation, we may be able to work with DUTCH test results, but serum blood hormone levels are the gold standard of testing to provide an accurate baseline. Many clinics offer finger prick tests, but we opt for serum blood tests as these are less likely to provide erroneous results and unnecessary additional costs if repeats needed. Once we begin treatment, the monitoring process is also much more efficient with serum blood tests.
Your Marion Gluck Clinic doctor will advise you of the tests required during your consultation and will explain the options for how you can have blood drawn.
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Nice Guidance On Menopause
In November 2015, NICE Guideline 23 on Menopause: diagnosis and management was published, providing recommendations for healthcare professionals who care for women in menopause.1 NICE NG23 also includes a section with information for the public, which provides women with information about what to expect from a healthcare professional if they have menopausal symptoms.
NICE Quality Standard 143,4 published in February 2017, distils the key points from NICE NG23 into measurable statements that can be used to assess the quality of care provided and quantify potential improvements to clinical practice. The five quality statements that make up NICE QS143 are listed in Table 1 and discussed in detail below.
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When Is The Best Time To Test Hormone Levels
If youre struggling with a hormone imbalance, testing your sex hormones can help you understand where the issue is rooted. Evaluating estrogen, progesterone and testosterone can be helpful in understanding which hormones are driving your symptoms.
Hormone levels arent static throughout the monthand this is why so many women get within range blood test results.
Use Your Symptoms As Your Guide
For more serious cases and symptoms, hormone testing can be very helpful. But for most women who experience the normal, if unpleasant, fluctuations of hormones during perimenopause and menopause, hormone tests are not generally needed â nor will they offer many answers. The best way to measure your hormones during perimenopause and menopause is to evaluate your symptoms. Take our quick hormonal profile to see how your symptoms rate.
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Fertility Hormone Testing Overview
- Fertility hormone testing usually involves analyzing a womans blood or urine samples to determine if hormonal issues may be preventing her from becoming pregnant.
- Hormonal imbalances are a leading cause of infertility in women.
- Hormone levels can also affect male fertility and hormone testing may be appropriate for men as well.
- Primary fertility hormone testing involves analysis of follicle-stimulating hormone , luteinizing hormone levels and progesterone.
What Happens During An Estrogen Test

Estrogens can be tested in blood, urine, or saliva. Blood or urine is usually tested in doctor’s office or lab. Saliva tests can be done at home.
For a blood test:
A health care professional will take a blood sample from a vein in your arm, using a small needle.
After the needle is inserted, a small amount of blood will be collected into a test tube or vial. You may feel a little sting when the needle goes in or out. This usually takes less than five minutes.
For a urine test:
Your health care provider may ask you to collect all urine passed in a 24-hour period. This is called a 24-hour urine sample test. For this test, your health care provider or a laboratory professional will give you a container to collect your urine and instructions on how to collect and store your samples. A 24-hour urine sample test generally includes the following steps:
- Empty your bladder in the morning and flush that urine down. Do not collect this urine. Record the time.
- For the next 24 hours, save all your urine passed in the container provided.
- Store your urine container in the refrigerator or a cooler with ice.
- Return the sample container to your health provider’s office or the laboratory as instructed.
For an at-home saliva test, talk your health care provider. He or she can tell you which kit to use and how to prepare and collect your sample.
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Which Tests Are Best For Hormonal Imbalance
Most companies that offer hormone test kits have several options. If you suspect a specific hormone imbalance, like a thyroid issue, you can order a test kit that focuses on those hormones. Otherwise, most companies offer a comprehensive test option that will check the levels of most or all of your major hormone types.
Check For Tsh Hormone Levels In Your Blood
TSH test or thyroid hormone stimulating test helps detect both hypothyroidism and hyperthyroidism. The TSH hormone is essential for your body as it instructs the gland on the quantity of T3 and T4 hormones that needs to be produced. TSH is produced in the pituitary gland of the brain. If your thyroid levels decrease, there is an excess production of TSH hormone. On the contrary, when there is high secretion of thyroid hormones, the pituitary gland secretes less TSH hormone into the blood.
A high TSH level seen in this hypothyroidism test indicates the presence of an underactive thyroid gland. On the other hand, if your thyroid gland is overactive, your TSH level will be low. However, in addition to this hypothyroidism blood test, you may need to undergo T3 and T4 tests to determine if there is a problem in your thyroid functioning. The normal TSH values in an individual range between 0.5-5.0 mIU/L .
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I suffered constant fatigue, daily stomach pain, arthritis, insomnia, and bowel movements 7-8 times a day that precluded me from doing what I wanted. I was on several daily medications. This program changed my life. I have much more energy, especially in the morning, I sleep much better, no more stomach pain, I can walk 9 miles now, and dont need any medications. This program gave me the opportunity to enjoy life, instead of constant struggle.
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Blood Tests For Infertility
If youre a woman who is 35 years old or younger and have been unable to conceive after one year of unprotected intercourse, or after six months if youre over 35, you and your partner should have afull physical and medical evaluation with a Reproductive Endocrinologist, a doctor who specializes in infertility diagnosis and treatment.
Several blood tests are available to determine if you, your partner, or both of you have a problem that is causing infertility.
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When Should You Start Fertility Testing
Your healthcare provider may begin a fertility evaluation if youve been trying to conceive for at least one year without success. But if youre older than 35, your healthcare provider may recommend a fertility evaluation after only six months of unprotected sex, says Shvetha Murthy Zarek, MD, a fertility specialist at Oma Fertility in St. Louis, Missouri. Having intercourse every other day is the optimal frequency to help sperm count stay high and increase the chance the sperm and egg will meet.
Diagnosis Of Womens Hormonal Imbalance
Since the symptoms of female hormone imbalance are also common signs of a number of other diseases and health conditions, women experiencing them should see a physician for a thorough physical examination. If other underlying medical conditions are ruled out, hormone level testing is the next step. Hormone levels can be measured through saliva testing and/or blood tests. Establishing an accurate diagnosis of hormone imbalance may require several tests, done at intervals, as hormone levels fluctuate throughout a womans menstrual cycle and may change greatly from one day to another.
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At What Age Should Amh Be Examined
Anti-Müllerian hormone is produced by the ovary, and its blood levels are an indicator of a woman’s ovarian reserve.
To ensure everything works as expected, we recommend that the first measurement is done from age 20 and not later than age 30. By doing this, if a woman has a diminished ovarian reserve at a young age, she would have time to decide whether she wants to have a baby now or cryopreserve some eggs to become a mother in the future.
When To Test Thyroid Labs

We cant discuss testing for hormone imbalances without looking at the thyroid. Thyroid testing is best down first thing in the morning and can be at any point in your cycle. We typically add these in when we are testing for other hormones.
Its important to stop any supplements with biotin at least 72 hours in advance as it can skew results.
At a minimum, I would suggest looking at:
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What Day Should Estrogen Levels Be Checked
When evaluating female estrogen levels, day 3 is when estradiol, along with follicle stimulating hormone and luteinizing hormone blood tests will be performed.
Testing at the beginning of the cycle, along with FSH can help us understand brain-ovarian communication. Measuring FSH and LH is important to assess how the brain is talking to the ovaries.
When Should FSH Levels Be Checked?
For fertility or evaluating ovarian reserve, FSH is tested on day 3 of your cycle. If you cannot have lab testing done, most providers are fine testing between days 2-4. Remember, day 1 is the first day of your period.
Testing on or around day three of your cycle can be helpful in evaluating PCOS diagnosis as well. Note, this test alone will not diagnose PCOS. FSH and LH can help us understand how the brain is talking to the ovaries and the hormones can help us know how the ovaries respond to that message.
Why Test Estradiol?
During your reproductive years, estradiol or E2 is the predominant form of estrogen. As we transition into menopause, estrone or E1 becomes the most common circulating estrogen. This is important to understand because depending on the phase of life you are in, your estrogen levels and the type of estrogen will vary.
If you are in a phase of life where you should be having periods, you need to check estradiol.
Testing Estrogen Days 19-22 of Cycle
Symptoms And Potential Complications
Imbalances in female hormones can have far-ranging effects on the body, producing a variety of physical, emotional and cognitive changes. These changes can cause symptoms that may include:
- Menstrual cycle changes, including shorter or longer times between periods, and periods that are longer, shorter, heavier or lighter than normal.
- Hot flashes and night sweats.
- Insomnia and other sleep disturbances
- Diminished sex drive
- Unexplained weight gain, especially in the abdominal area, hips and thighs
- Skin changes, such as thinning, dryness and wrinkling
- Dry, brittle hair
Female hormone imbalance can increase a womans risk of a number of diseases and health problems. Among the most serious of these is heart disease, with risk increasing as estrogen levels decrease. Women with hormonal imbalances are also at greater risk for osteoporosis, since low levels of estrogens can interfere with the absorption of calcium and other nutrients essential to the maintenance of bone health and density.
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